eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
Current issue Archive Manuscripts accepted About the journal Editorial board Abstracting and indexing Subscription Contact Instructions for authors Ethical standards and procedures
SCImago Journal & Country Rank
2/2020
vol. 45
 
Share:
Share:
more
 
 
abstract:
Clinical immunology

Non-classical human leukocyte antigen class I in Tunisian children with autism

Najla Kharrat
1
,
Rania Abdelhedi
1
,
Imen Gtif
1
,
Imen Ayadi
1
,
Roberta Rizzo
1
,
Daria Bortolotti
1
,
Nouha Bouayed Abdelmoula
2
,
Farhat Ghribi
1, 3
,
Ahmed Rebai
1
,
Ines Zidi
4

1.
Laboratory of Molecular and Cellular Screening Process, Centre of Biotechnology of Sfax, Sfax University, Tunisia
2.
Research Unit: UR17ES36 Genomics of Signalopathies in the Service of Medicine, Sfax University, Medical University of Sfax, Tunisia
3.
Department of Child and Adolescent Psychiatry, Hédi Chaker University Hospital, Sfax, Tunisia
4.
Laboratory Microorganismes and Active Biomolecules, Sciences Faculty of Tunis, University of Tunis El Manar, Tunis, Tunisia
Cent Eur J Immunol 2020; 45 (2): 176-183
Online publish date: 2020/07/27
View full text
Get citation
ENW
EndNote
BIB
JabRef, Mendeley
RIS
Papers, Reference Manager, RefWorks, Zotero
AMA
APA
Chicago
Harvard
MLA
Vancouver
 
Autism spectrum disorders (ASD) are one of the most common childhood morbidities characterized by deficits in communication and social skills. Increasing evidence has suggested associations between immune genes located in the human leukocyte antigen (HLA) complex and etiology of autism.

In this study, we investigated whether the non-classical class I HLA-G, -E, and -F polymorphisms are associated with genetic predisposition to autism in Tunisia. We aimed to find a correlation between HLA-G genotypes and soluble HLA-G (sHLA-G) levels. We have analyzed the HLA-G, -E, and -F genotypes of 15 autistic children and their parents. DNA typing of HLA class I genes was performed using PCR-SSP and PCR-RFLP methods. Also, we evaluated the serum levels of HLA-G (1 and 5) by a validated ELISA technique in autistic probands and their parents.

No association was found between any polymorphism and autism in the study subjects. Additionally, we found no correlation between sHLA-G1 and sHLA-G5 and autism. Also, no significant difference in sHLA-G testing in parents and offspring was found. However, parents carrying [GG] genotype presented a higher sHLA-G levels than those carrying ([CC]+[GC]) genotypes (p = 0.037).

From this preliminary study, we conclude that the investigated polymorphisms of HLA-G, -E, and -F genes did not lead to autism susceptibility in Tunisian children. However, the CGTIGA haplotype was found to be associated with the disease.
keywords:

polymorphism, autism, HLA-G, HLA-E, HLA-F

Quick links
© 2020 Termedia Sp. z o.o. All rights reserved.
Developed by Bentus.
PayU - płatności internetowe