Abstract
2/2013
vol. 30
Original paper
CATECHOLAMINES AND β2-ADRENOCEPTOR
GENE EXPRESSION BEFORE AND AFTER
MAXIMAL INCREMENTAL CYCLE TEST IN
YOUNG ICE HOCKEY PLAYERS: RELATION
TO WORK PERFORMED
Biol. Sport 2013;30:85-90
Online publish date: 2014/07/22
The aim of this study was to assess the plasma adrenaline and noradrenaline concentrations as well
as whole blood β2-adrenoceptor gene (ADRB2) expression in young ice hockey players before and immediately
after exercise in relation to performed work. Nineteen Youth National Team ice hockey players were subjected
to the maximal incremental cycloergometer exercise. The test was done in the pre-competitive phase of training.
Among many parameters the plasma adrenaline and noradrenaline concentrations and ADRB2 gene expression
in peripheral blood mononuclear cells (PBMC) were determined before and after exercise. The average performed
work was 3261.3 ± 558.3 J · kg-1 and maximal oxygen consumption (VO2max) for all players was 53.85 ± 3.91
mL · kg-1 min-1. The geometric mean of the ADRB2 gene expression was statistically significantly different before
and after exercise (P ≤ 0.05), while adrenaline and noradrenaline levels in plasma significantly increased after
exercise. In the analysed group of athletes we found that initial level of plasma noradrenaline correlated with
the performed work (r = - 0.55, P < 0.014) and normalized ADRB2 expression before the exercise correlated
with the work done by them (r = 0.48, P<0.039). However, no statistically significant correlations were found
between the plasma adrenaline or noradrenaline concentrations and ADRB2 gene expression in peripheral blood
of the players. The performed work in the maximal incremental exercise test of regularly training young ice hockey
players depends on the initial levels of noradrenaline in plasma and ADRB2 mRNA in PBMC.
as whole blood β2-adrenoceptor gene (ADRB2) expression in young ice hockey players before and immediately
after exercise in relation to performed work. Nineteen Youth National Team ice hockey players were subjected
to the maximal incremental cycloergometer exercise. The test was done in the pre-competitive phase of training.
Among many parameters the plasma adrenaline and noradrenaline concentrations and ADRB2 gene expression
in peripheral blood mononuclear cells (PBMC) were determined before and after exercise. The average performed
work was 3261.3 ± 558.3 J · kg-1 and maximal oxygen consumption (VO2max) for all players was 53.85 ± 3.91
mL · kg-1 min-1. The geometric mean of the ADRB2 gene expression was statistically significantly different before
and after exercise (P ≤ 0.05), while adrenaline and noradrenaline levels in plasma significantly increased after
exercise. In the analysed group of athletes we found that initial level of plasma noradrenaline correlated with
the performed work (r = - 0.55, P < 0.014) and normalized ADRB2 expression before the exercise correlated
with the work done by them (r = 0.48, P<0.039). However, no statistically significant correlations were found
between the plasma adrenaline or noradrenaline concentrations and ADRB2 gene expression in peripheral blood
of the players. The performed work in the maximal incremental exercise test of regularly training young ice hockey
players depends on the initial levels of noradrenaline in plasma and ADRB2 mRNA in PBMC.
Keywords
ice hockey players, catecholamines, ADRB2, maximal incremental cycle test
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