Abstract
1/2012
vol. 29
Original paper
TIME COURSE ALTERATIONS OF SATELLITE
CELL EVENTS IN RESPONSE TO LIGHT
MODERATE ENDURANCE TRAINING
IN WHITE GASTROCNEMIUS MUSCLE OF
THE RAT
Biol. Sport 2012;29:51-56
Online publish date: 2014/08/22
This study investigated satellite cells and their related molecular events adapted to light moderate
endurance training in the white gastrocnemius muscle of the rat. The white gastrocnemius muscle of male
Sprague-Dawley rats that had been trained for 4 weeks and 8 weeks, with control rats being analysed alongside
them, was selected for analysis (n=3 per group). The training protocol consisted of treadmill running at
20 m · min-1 for 30 min on a 0% grade, for 3 days · week-1. Immunohistochemical staining coupled with image
analysis was used for quantification. To provide deeper insight into the cell layer, 40 sections per rat, corresponding
to 120 values per group, were obtained as a mean value for statistical comparison. The results indicated that
at week 4, training effects increased the vascular endothelial growth factor (VEGF) content and c-met positive
satellite cell numbers. At week 8, the training effect was attenuated for VEGF and c-met satellite cell numbers,
but it increased in the muscle fibre area. Additionally, c-met positive satellite cell numbers correlated with VEGF
content (r = 0.79, p<0.05). In conclusion, this study suggests that light moderate endurance training could
stimulate satellite cell activation that might be related to VEGF signalling. Additionally, the satellite cells activated
by moderate endurance training might contribute to slight growth in myocytes.
endurance training in the white gastrocnemius muscle of the rat. The white gastrocnemius muscle of male
Sprague-Dawley rats that had been trained for 4 weeks and 8 weeks, with control rats being analysed alongside
them, was selected for analysis (n=3 per group). The training protocol consisted of treadmill running at
20 m · min-1 for 30 min on a 0% grade, for 3 days · week-1. Immunohistochemical staining coupled with image
analysis was used for quantification. To provide deeper insight into the cell layer, 40 sections per rat, corresponding
to 120 values per group, were obtained as a mean value for statistical comparison. The results indicated that
at week 4, training effects increased the vascular endothelial growth factor (VEGF) content and c-met positive
satellite cell numbers. At week 8, the training effect was attenuated for VEGF and c-met satellite cell numbers,
but it increased in the muscle fibre area. Additionally, c-met positive satellite cell numbers correlated with VEGF
content (r = 0.79, p<0.05). In conclusion, this study suggests that light moderate endurance training could
stimulate satellite cell activation that might be related to VEGF signalling. Additionally, the satellite cells activated
by moderate endurance training might contribute to slight growth in myocytes.
Keywords
c-met, exercise, VEGF
Integrated with
Editorial Policies
