eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
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SCImago Journal & Country Rank
4/2020
vol. 45
 
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abstract:
Clinical immunology

Regulatory effect of daphnetin on the balance of Th17 and Treg cells in the peripheral blood mononuclear cells from patients with unexplained recurrent pregnancy loss

Zhiqin Zhang
1
,
Shenggen Long
2
,
Zhihui Huang
3
,
Jun Tan
2
,
Qiongfang Wu
2
,
Ouping Huang
4

1.
Department of Human Assisted Reproductive, Maternal and Child Health Affiliated Hospital of Nanchang University, Nanchang City, Jiangxi, PR China
2.
Department of Human Assisted Reproductive, Jiangxi Maternity and Child Healthcare Hospital, Nanchang City, Jiangxi, PR China
3.
Jiangxi Maternity and Child Healthcare Hospital, Nanchang City, Jiangxi, PR China
4.
Department of Gynecology, Jiangxi Maternity and Child Healthcare Hospital, Nanchang City, Jiangxi, PR China
Centr Eur J Immunol 2020; 45 (4): 403-408
Online publish date: 2021/01/30
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Introduction
There has a close relationship between the balance of T helper 17 (Th17) cells and Foxp3+ regulatory T cells (Treg) and unexplained recurrent pregnancy loss (URPL). The present study is to investigate the regulatory effect of daphnetin, which is extracted from Daphne odora Var, on the balance of Th17 and Treg cells in the peripheral blood mononuclear cells (PBMCs) from patients with URPL.

Material and methods
PBMCs were isolated from 35 pregnant women with URPL and 35 women with normal early pregnancies, respectively and treated with daphnetin for three days. Flow cytometry was performed to measure the proportions of Th17 and Treg cells. The level of expression of IL-6, TGF-b1 and IL-2 were detected using enzyme-linked immunosorbent assay (ELISA) and the level of expression of FoxP3, RORgt, signal transducers and activators of transcriotion 3 (STAT3) and STAT5 were detected by RT-PCR.

Results
The concentrations of Th17-type cytokines IL-2 were significantly decreased in the URPL group after treatment (p < 0.01). Treg-type cytokines such as TGF-b1 and IL-6 were significantly increased after treatment (p < 0.01). At the same time, daphnetin may induce a decrease in the ratio of RORgt to Foxp3 and a Treg cell bias, which would be beneficial for pregnancy maintenance. Futhermore the expression level of STAT3 were higher in the URPL patients whereas STAT5 were lower than those in the control subjects.

Conclusions
In conclusion, daphnetin may have regulatory effect on the balance of Th17 and Treg cells in the peripheral blood mononuclear cells from patients with URPL.

keywords:

daphnetin, unexplained recurrent pregnancy loss, Th17 cells, Treg cells, peripheral blood mononuclear cells

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