Structure and antigenicity analysis of the IgG gene for <i>Nyctereutes procyonoides</i>

Cui Zhao; Shuyuan Guo; Xiaoru Pang; Daozhen Song; Shijun Fu; Weishan Chang

doi:10.5114/ceji.2015.54585

eISSN: 1644-4124
ISSN: 1426-3912

Central European Journal of Immunology

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3/2015
vol. 40

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abstract:

Experimental immunology

Structure and antigenicity analysis of the IgG gene for Nyctereutes procyonoides

Cui Zhao

,

Shuyuan Guo

,

Xiaoru Pang

,

Daozhen Song

,

Shijun Fu

,

Weishan Chang

(Cent Eur J Immunol 2015; 40 (3): 271-275)

DOI: https://doi.org/10.5114/ceji.2015.54585

Online publish date: 2015/10/15

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Objective: Nyctereutes procyonoides immunoglobulin G (IgG) gene is partially cloned.

Material and methods: In order to obtain a certain length (966bp) of Nyctereutes procyonoides immunoglobulin G (IgG), two pairs of primers are designed according to the conserved nucleotide sequence of canine (GenBank:AF354265, AF354265, AF354266, AF354267) and mink (GenBank: L07789). Using Bioinformatics technology and Western-blot to analyze antigenicity of Nyctereutes procyonoides IgG-B gene.

Results: The homology for nucleotide sequence of IgG between Nyctereutes procyonoides and canine (IgG A, IgG B, IgG C, IgG D), mink, Homo sapiens, Oryctolagus cuniculus, Mus musculus, Anas platyrhynchos and gallus were respectively (88.1%, 93.6%, 85.4%, 87.2%), 83.7%, 74.8%, 71.8%, 69.2%, 51.6%, 48.4%. It can be seen that there was high homology of aminoacid sequence between IgG of Nyctereutes procyonoides and IgG (A, B, C, D) of canine. And the serum antibody of Nyctereutes procyonoides had obviously cross-reaction with HRP conjugated rabbit anti-dog IgG,compared with those of canine, oryctolagus cuniculus, mus musculus, mink, gallus.

Conclusions: We successfully got Nyctereutes procyonoides immuneglobulin G (IgG) gene (GenBank: KM010191). There is the closest ties of consanguinity of IgG exist between Nyctereutes procyonoides and canine among the mammal through the genetic evolution.The detection and treament of canine distemper can be used on Nyctereutes procyonoides.

keywords:

Structure and antigenicity analysis of the IgG gene for Nyctereutes procyonoides

Cui Zhao , Shuyuan Guo , Xiaoru Pang , Daozhen Song , Shijun Fu , Weishan Chang

gene cloning, western blot, genetic evolution analysis, Nyctereutes procyonoides, IgG

Cui Zhao

,

Shuyuan Guo

,

Xiaoru Pang

,

Daozhen Song

,

Shijun Fu

,

Weishan Chang