Comparative Study of Potassium Hydroxide, Parker Ink
and Chicago Sky Blue for Diagnosing Dermatophytosis

Vikas Malgotra; Mashkoor Wani; Deepti Gupta

doi:10.5114/dr.2025.160256

eISSN: 2084-9893
ISSN: 0033-2526

Dermatology Review/Przegląd Dermatologiczny

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Otwarty dostęp

6/2025
vol. 112

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Artykuł oryginalny

Comparative Study of Potassium Hydroxide, Parker Ink and Chicago Sky Blue for Diagnosing Dermatophytosis

Vikas Malgotra

¹

,

Mashkoor Wani

¹

,

Deepti Gupta

²

Dermatology Department, Government Medical College, Jammu, India
Pathology Department, Government Medical College, Jammu, India

Dermatol Rev/Przegl Dermatol 2025, 112, 349-354

DOI: https://doi.org/10.5114/dr.2025.160256

Data publikacji online: 2026/02/28

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Metryki PlumX:

Introduction
Superficial fungal infections affect approximately 20–25% of the global population, with dermatophytosis representing the most common form. Although diagnosis is often based on clinical presentation, laboratory confirmation is frequently required. Direct microscopic examination with potassium hydroxide is widely used; however, its diagnostic performance may vary. Alternative staining techniques may enhance fungal visualization.

Objective
To compare the diagnostic performance of potassium hydroxide, Parker ink staining, and Chicago sky blue staining in patients with dermatophytosis.

Material and methods
In this prospective observational study, skin scrapings, nail scrapings, and hair samples from patients with clinically suspected dermatophytosis were examined using the three staining methods.

Results and conclusions
Chicago sky blue demonstrated the highest sensitivity and specificity, followed by Parker ink and potassium hydroxide. This method also showed the best predictive values and overall diagnostic accuracy, indicating superior performance and potential usefulness in routine clinical practice.

Indeks osobowy

Comparative Study of Potassium Hydroxide, Parker Ink and Chicago Sky Blue for Diagnosing Dermatophytosis

Vikas Malgotra 1 , Mashkoor Wani 1 , Deepti Gupta 2

Vikas Malgotra

¹

,

Mashkoor Wani

¹

,

Deepti Gupta

²