eISSN: 1509-572x
ISSN: 1641-4640
Folia Neuropathologica
Current issue Archive Manuscripts accepted About the journal Abstracting and indexing Subscription Contact Instructions for authors
SCImago Journal & Country Rank
1/2018
vol. 56
 
Share:
Share:
more
 
 
abstract:
Original paper

Degenerative effect of Ankaferd Blood Stopper® on mice peripheral sensory neurons in vitro

Ramazan Üstün, Elif Kaval Oğuz

Folia Neuropathol 2018; 56 (1): 67-74
Online publish date: 2018/03/28
View full text
Get citation
ENW
EndNote
BIB
JabRef, Mendeley
RIS
Papers, Reference Manager, RefWorks, Zotero
AMA
APA
Chicago
Harvard
MLA
Vancouver
 
Ankaferd Blood Stopper® (ABS) is a licensed medicinal herbal extract that ensures effective hemostasis on external, internal, postoperative and dental bleeds. Dorsal root ganglia (DRG) harbor cell bodies of peripheral sensory neurons. DRG neurons receive peripheral information and regularly send projections to nuclei in the brainstem and the spinal cord. These neurons play critical roles in neural development. Neuronal dysfunctions were reported due to ABS use in surgical interventions. The purpose of this experiment was to investigate the degenerative effects of the ABS on mice DRG cells in vitro. DRG neurons were isolated from adult mice and cultured in vitro. The neurons were incubated with various concentrations of ABS for 24 h. At the end of 24 hours, under fluorescence microscopy, cell viability was determined with the fluorescent dye calcein-AM, and cell death was determined with the fluorescent dye propidium iodide. The behavior of the cells was displayed with time-lapse video microscopy for 12 hours from the time of treatment. ABS killed both neurons and non-neuronal cells via necrosis at a concentration of 25 µl/ml or more. ABS has the degenerative effect on mice peripheral sensory neurons, depending on the ABS level.
keywords:

DRG neurons, Ankaferd Blood Stopper, degeneration, cell death, in vitro

references:
Antoine JC, Robert-Varvat F, Maisonobe T, Créange A, Franques J, Mathis S, Delmont E, Kuntzer T, Lefaucheur JP, Pouget J et al. Identifying a therapeutic window in acute and subacute inflammatory sensory neuronopathies. J Neurol Sci 2016; 361: 187-191.
Berta T, Park CK, Xu ZZ, Xie RG, Liu T, Lu N, Liu YC, Ji RR. Extracellular caspase-6 drives murine inflammatory pain via microglial TNF-alpha secretion. J Clin Invest 2014; 124: 1173-1186.
Bilgili H, Captug O, Kosar A, Kurt M, Kekilli M, Shorbagi A, Kurt OK, Ozdemir O, Goker H, Haznedaroglu IC. Oral systemic administration of Ankaferd blood stopper has no short-term toxicity in an in vivo rabbit experimental model. Clin Appl Thromb Hemost 2010; 16: 533-536.
Dincol ME, Ozbas H, Yilmaz B, Ersev H, Gokyay S, Olgac V. Effect of the plant-based hemostatic agent Ankaferd Blood Stopper(R) on the biocompatibility of mineral trioxide aggregate. BMC Oral Health 2016; 16: 1-9.
Drug I Immun Drug Cosmetic Co. http://www.ankaferd.com.tr/. Accessed May 29, 2017.
Emes Y, Aybar B, Vural P, Issever H, Yalcn S, Atalay B, Dincol E, Bilir A. Effects of hemostatic agents on fibroblast cells. Implant Dent 2014; 23: 641-647.
Evren C, Ugur MB, Yildirim B, Bektas S, Yigit VB, Cinar F. Unpredicted effects of Ankaferd(R) on cartilage tissue. Int J Clin Exp Med 2015; 8: 922-927.
Garber A, Jang S. Novel Therapeutic Strategies in the Management of Non-Variceal Upper Gastrointestinal Bleeding. Clin Endosc 2016; 49: 421-424.
Gazzeri R, Galarza M, Callovini G, Alfieri A. Biosurgical Hemostatic Agents in Neurosurgical Intracranial Procedures. Surg Technol Int 2017; 30: 468-476.
Goker H, Haznedaroglu IC, Ercetin S, Kirazli S, Akman U, Ozturk Y, Firat HC. Haemostatic actions of the folkloric medicinal plant extract Ankaferd Blood Stopper. J Int Med Res 2008; 36: 163-170.
Graham RK, Ehrnhoefer DE, Hayden MR. Caspase-6 and neurodegeneration. Trends Neurosci 2011; 34: 646-656.
Gwathmey KG. Sensory neuronopathies. Muscle Nerve 2016; 53: 8-19.
Haznedaroglu BZ, Beyazit Y, Walker SL, Haznedaroglu IC. Pleiotropic cellular, hemostatic, and biological actions of Ankaferd hemostat. Crit Rev Oncol Hematol 2012; 83: 21-34.
Huri E, Akgul T, Ayyildiz A, Bagcioglu M, Germiyanoglu C. First clinical experience of Ankaferd BloodStopper as a hemostatic agent in partial nephrectomy. Kaohsiung J Med Sci 2010; 26: 493-495.
Huri E, Akgul T, Ayyildiz A, Germiyanoglu C. Hemostasis in retropubic radical prostatectomy with Ankaferd BloodStopper: a case report. Kaohsiung J Med Sci 2009; 25: 445-447.
Kaya I, Gulabi D, Yilmaz M, Bas A, Cecen GS, Sener N. Intraarticular Ankaferd blood stopper application increases cartilagedegeneration: an experimental study. Turk J Med Sci 2016; 46: 236-240.
Kurt M, Onal I, Akdogan M, Kekilli M, Arhan M, Sayilir A, Oztas E, Haznedaroglu I. Ankaferd Blood Stopper for controlling gastrointestinal bleeding due to distinct benign lesions refractory to conventional antihemorrhagic measures. Can J Gastroenterol 2010; 24: 380-384.
Meric Teker A, Korkut AY, Kahya V, Gedikli O. Prospective, randomized, controlled clinical trial of Ankaferd Blood Stopper in patients with acute anterior epistaxis. Eur Arch Otorhinolaryngol 2010; 267: 1377-1381.
Mihmanli A, Ulker Z, Alpsoy L, Ezirganli S. Evaluation of cytotoxicity of a new hemostatic agent Ankaferd Blood Stopper(R) using different assays. Hum Exp Toxicol 2012; 31: 780-787.
Mumcuoglu M, Akin DF, Ezer U, Akar N. Ankaferd Blood Stopper induces apoptosis and regulates PAR1 and EPCR expression in human leukemia cells. Egyptian Journal of Medical Human Genetics 2015; 16: 19-27.
Nikolaev A, McLaughlin T, O’Leary DD, Tessier-Lavigne M. APP binds DR6 to trigger axon pruning and neuron death via distinct caspases. Nature 2009; 457: 981-989.
Odabas ME, Erturk M, Cinar C, Tuzuner T, Tulunoglu O. Cytotoxicity of a new hemostatic agent on human pulp fibroblasts in vitro. Med Oral Patol Oral Cir Bucal 2011; 16: e584-587.
Ozaslan E, Purnak T, Yildiz A, Haznedaroglu IC. The effect of a new hemostatic agent for difficult cases of non-variceal gastrointestinal bleeding: Ankaferd blood stopper. Hepatogastroenterology 2010; 57: 191-194.
Ozturk G, Cengiz N, Erdogan E, Him A, Oguz EK, Yenidunya E, Aysit N. Two distinct types of dying back axonal degeneration in vitro. Neuropathol Appl Neurobiol 2013; 39: 362-376.
Pampu AA, Yildirim M, Tuzuner T, Baygin O, Abidin I, Dayisoylu EH, Senel FC. Comparison of the effects of new folkloric hemostatic agent on peripheral nerve function: an electrophysiologic study in rats. Oral Surg Oral Med Oral Pathol Oral Radiol 2013; 115: e1-6.
Pamuk F, Cetinkaya BO, Keles GC, Balli U, Koyuncuoglu CZ, Cintan S, Kantarci A. Ankaferd blood stopper enhances healing after osseous grafting in patients with intrabony periodontal defects. J Periodontal Res 2016; 51: 540-547.
Sanchez-Migallon MC, Valiente-Soriano FJ, Nadal-Nicolas FM, Vidal-Sanz M, Agudo-Barriuso M. Apoptotic Retinal Ganglion Cell Death After Optic Nerve Transection or Crush in Mice: Delayed RGC Loss With BDNF or a Caspase 3 Inhibitor. Invest Ophthalmol Vis Sci 2016; 57: 81-93.
Siironen J, Sandberg M, Vuorinen V, Roytta M. Expression of type I and III collagens and fibronectin after transection of rat sciatic nerve. Reinnervation compared with denervation. Lab Invest 1992; 67: 80-87.
Teker AM, Korkut AY, Gedikli O, Kahya V. Prospective, controlled clinical trial of Ankaferd Blood Stopper in children undergoing tonsillectomy. Int J Pediatr Otorhinolaryngol 2009; 73: 1742-1745.
Turk S, Malkan UY, Ghasemi M, Hocaoglu H, Mutlu D, Gunes G, Aksu S, Haznedaroglu IC. Growth inhibitory activity of Ankaferd hemostat on primary melanoma cells and cell lines. SAGE Open Med 2017; 5: 1-7.
Ugur A, Sarac N, Cankal DA, Ozle M. The antioxidant and antimutagenic activities of Ankaferd blood stopper,a natural hemostatic agent used in dentistry. Turk J Med Sci 2016; 46: 657-663.
Vezeau PJ. Topical Hemostatic Agents: What the Oral and Maxillofacial Surgeon Needs to Know. Oral Maxillofac Surg Clin North Am 2016; 28: 523-532.
Zheng X, Chen F, Zheng T, Huang F, Chen J, Tu W. Amitriptyline Activates TrkA to Aid Neuronal Growth and Attenuate Anesthesia-Induced Neurodegeneration in Rat Dorsal Root Ganglion Neurons. Medicine (Baltimore) 2016; 95: e3559.
Quick links
© 2018 Termedia Sp. z o.o. All rights reserved.
Developed by Bentus.
PayU - płatności internetowe