eISSN: 1509-572x
ISSN: 1641-4640
Folia Neuropathologica
Current issue Archive Manuscripts accepted About the journal Abstracting and indexing Subscription Contact Instructions for authors
SCImago Journal & Country Rank
vol. 56
Original paper

Effect of accumbens nucleus shell lesioning on bitemporal lobe epilepsy in rat model

Junwu Fu, Yawei Liu, Kaijun Yang, Hao Long, Kewan Wang, Songtao Qi

Folia Neuropathol 2018; 56 (4): 346-353
Online publish date: 2018/12/31
View full text
Get citation
JabRef, Mendeley
Papers, Reference Manager, RefWorks, Zotero
To explore the effect of accumbens nucleus shell (ACbSh) lesioning on bitemporal lobe epilepsy.

Material and methods
Adult Wistar rats (male) were enrolled and randomly assigned into the control group and epilepsy groups with multiple time-points. Lithium-pilocarpine was used to establish the rat epilepsy model, while the control group received an equal amount of saline. Ibotenic acid stereotaxic injection was performed to cause accumbens nucleus shell lesioning for specific groups. Cascade software was used for electroencephalogram (EEG) examination. Fluoro-Jade C staining was performed to examine neuronal degeneration.

Latency period of the epilepsy in epilepsy groups was 15.3 ± 1.1 min, and epilepsy intensity was 4.8 ± 0.5 events/ 12 h. ACbSh lesioning significantly reduced aggressive behavior. Compared with epilepsy groups without ACbSh lesioning, ACbSh lesioning significantly decreased epileptic seizures and reduced epileptic duration (p < 0.05). EEG showed that there were still sharp waves in the hippocampus and amygdala region after ACbSh lesioning, but epileptic discharge in prefrontal cortex was significantly decreased (p < 0.05), while epilepsy groups without ACbSh lesioning had more sharp waves in the prefrontal cortex, hippocampus and amygdala region. Fluoro-Jade C staining showed that ACbSh lesioning significantly decreased grades of neuronal degeneration (p < 0.05).

Recurrent epilepsy caused neuronal degeneration via ACbSh region-related pathways, and ACbSh lesioning could mitigate epilepsy-caused neuronal degeneration by reducing epileptic discharge.


epilepsy, accumbens nucleus shell, electrophysiology, neuronal degeneration

Bedwell J, Gooding D, Chan C, Trachik B. Anhedonia in the age of RDoC. Schizophr Res 2014; 160: 226-227.
Bonilha L, Helpern J, Sainju R, Nesland T, Edwards J, Glazier S, Tabesh A. Presurgical connectome and postsurgical seizure control in temporal lobe epilepsy. Neurology 2013; 81: 1704-1710.
Chen L, Feng H, Mao X, Ye Q, Zeng L. One hour of pilocarpineinduced status epilepticus is sufficient to develop chronic epilepsy in mice, and is associated with mossy fiber sprouting but not neuronal death. Neurosci Bull 2013; 29: 295-302.
Chen N, Yan N, Liu C, Ge Y, Zhang JG, Meng FG. Neuroprotective effects of electrical stimulation of the anterior nucleus of the thalamus for hippocampus neurons in intractable epilepsy. Medical Hypotheses 2013; 80: 517-519.
ElBatsh M, Assareh N, Marsden C, Kendall D. Anxiogenic-like effects of chronic cannabidiol administration in rats. Psychopharmacology (Berl) 2012; 221: 239-247.
Engel J Jr, McDermott MP, Wiebe S, Langfitt JT, Stern JM, Dewar S, Sperling MR, Gardiner I, Erba G, Fried I, Jacobs M, Vinters HV, Mintzer S, Kieburtz K. Early surgical therapy for drug-resistant temporal lobe epilepsy: a randomized trial. JAMA 2012; 307: 922-930.
Fisher R, Acevedo C, Arzimanoglou A, Bogacz A, Cross J, Elger C, Engel J Jr, Forsgren L, French J, Glynn M, Hesdorffer D, Lee B, Mathern G, Moshé S, Perucca E, Scheffer IE, Tomson T, Watanabe M, Wiebe S. ILAE official report: a practical clinical definition of epilepsy. Epilepsia 2014; 55: 475-482.
Ge Y, Hu W, Liu C, Zhang JG, Meng FG. Brain stimulation for treatment of refractory epilepsy. Chinese Medical Journal 2013; 126: 3364-3370.
Goodwin G, Yacko H. Emergence of the exploratory motive in rats. Dev Psychobiol 2004; 45: 34-48.
Guimaraes F, Chiaretti T, Graeff F, Zuardi A. Antianxiety effect of cannabidiol in the elevated plus-maze. Psychopharmacology (Berl) 1990; 100: 558-559.
Lee K, Shon Y, Cho C. Long-term outcome of anterior thalamic nucleus stimulation for intractable epilepsy. Stereotactic and Functional Neurosurgery 2012; 90: 379-385.
Lee Y, Oh JS, Chung SJ, Chung SJ, Kim SJ, Nam CM, Lee PH, Kim JS, Sohn YH. Does smoking impact dopamine neuronal loss in de novo Parkinson disease? Ann Neurol 2017; 82: 850-854.
Leweke F, Piomelli D, Pahlisch F, Muhl D, Gerth C, Hoyer C. Symptoms of schizophrenia. Transl Psychiatry 2012; 2: e94.
Li N, Wang J, Wang XL, Chang CW, Ge SN, Gao L, Wu HM, Zhao HK, Geng N, Gao GD. Nucleus accumbens surgery for addiction. World Neurosurg 2013; 80: S28.e9-19.
Min B, Guoming L, Jian Z. Treatment of mesial temporal lobe epilepsy with amygdalohippocampal stimulation: A case series and review of the literature. Exp Ther Med 2013; 5: 1264-1268.
Moreira F, Guimaraes F. Cannabidiol inhibits the hyperlocomotion induced by psychotomimetic drugs in mice. Eur J Pharmacol 2005; 512: 199-205.
Moshé SL, Perucca E, Ryvlin P, Tomson T. Epilepsy: new advances. Lancet 2015; 385: 884-898.
Pellow S, Chopin P, File SE, Briley M. Validation of open: closed arm entries in an elevated plus-maze as a measure of anxiety in the rat. J Neurosci Methods 1985; 14: 149-167.
Richardson M. Large scale brain models of epilepsy: dynamics meets connectomics. J Neurol Neurosurg Psychiatry 2012; 83: 1238-1248.
Schmitt F, Voges J, Heinze H, Zaehle T, Holtkamp M, Kowski A. Safety and feasibility of nucleus accumbens stimulation in five patients with epilepsy. J Neurol 2014; 261: 1477-1484.
Serletis D, Bulacio J, Bingaman W, Najm I, González-Martínez J. The stereotactic approach for mapping epileptic networks: a prospective study of 200 patients. J Neurosurg 2014; 121: 1239-1246.
Shoval G, Shbiro L, Hershkovitz L, Hazut N, Zalsman G, Mechoulam R, Weller A. Prohedonic Effect of Cannabidiol in a Rat Model of Depression. Neuropsychobiology 2016; 73: 123-129.
Sprengers M, Vonck K, Carrette E, Marson AG, Boon P. Deep brain and cortical stimulation for epilepsy. Cochrane Database Syst Rev 2014; 6: CD008497.
Stam CJ. Modern network science of neurological disorders. Nature reviews. Neuroscience 2014; 15: 683-695.
Tykocki T, Mandat T, Kornakiewicz A, Koziara H, Nauman P. Deep brain stimulation for refractory epilepsy. Arch Med Sci 2012; 8: 805-816.
Vinod K, Xie S, Psychoyos D, Hungund B, Cooper T, Tejani-Butt S. Dysfunction in fatty acid amide hydrolase is associated with depressive-like behavior in Wistar Kyoto rats. PLoS One 2012; 7: e36743.
Quick links
© 2019 Termedia Sp. z o.o. All rights reserved.
Developed by Bentus.
PayU - płatności internetowe