eISSN: 1509-572x
ISSN: 1641-4640
Folia Neuropathologica
Current issue Archive Manuscripts accepted About the journal Abstracting and indexing Subscription Contact Instructions for authors
SCImago Journal & Country Rank
vol. 55
Original paper

Effects of green tea epigallocatechin-3-gallate on the proteolipid protein and oligodendrocyte transcription factor 1 messenger RNA gene expression in a mouse model of multiple sclerosis

Mohammadreza Semnani, Farhad Mashayekhi, Mahnaz Azarnia, Zivar Salehi

Folia Neuropathol 2017; 55 (3): 199-205
Online publish date: 2017/09/30
View full text
Get citation
JabRef, Mendeley
Papers, Reference Manager, RefWorks, Zotero
The cuprizone multiple sclerosis (MS) animal model is characteristic for toxic demyelination and represents a reversible demyelination and remyelination system. It has been shown that green tea epigallocatechin-3-gallate (EGCG) might be effective in improving the symptoms and pathological conditions associated with autoimmune inflammatory diseases in several animal models. In this study the effects of EGCG on proteolipid protein (PLP) and oligodendrocyte transcription factor 1 (Olig1) expression in the cerebral cortex of a murine model of cuprizone-induced demyelination was investigated. C57BL/6 mice were treated with cuprizone for six weeks in order to induce demyelination. Immediately after the cessation of cuprizone the animals were divided into 6 groups (n = 10 for each group). The first two groups were injected intraperitoneally (IP) with EGCG in the amount of 50 mg/kg/daily body weight for 2 and 4 weeks. The second two groups (SHAM) were injected IP with phosphate-buffered saline (PBS) for 2 and 4 weeks, and the third two groups were left without injection as controls. After two and four weeks the mice were killed and the cerebral cortex was collected and the expression of Plp and Olig1 was studied by real-time PCR. The results showed significant increases in PLP and Olig1 expression in the EGCG-treated groups as compared to the SHAM and control groups (p < 0.0001). It is concluded that EGCG increases PLP and Olig1 expression in the cerebral cortex of a mouse model of MS induced by cuprizone.

cuprizone, EAE, EGCG, Plp, Olig1

Aktas O, Prozorovski T, Smorodchenko A, Savaskan NE, Lauster R, Kloetzelm PM, InfanteDuarte C, Brocke S, Zipp F. Green tea epigallocatechin-3-gallate mediates T cellular NF-kappa B inhibition and exerts neuroprotection in autoimmune encephalomyelitis. J Immunol 2004; 173: 5794-5800.
Balentine DA, Wiseman SA, Bouwens LC. The chemistry of tea flavonoids. Food Sci Nutr 1997; 37: 693-704.
Cammer W. The neurotoxicant, cuprizone, retards the differentiation of oligodendrocytes in vitro. J Neurol Sci 1999; 168: 116-120.
Costenbader KH, Gay S, Alarcón-Riquelme ME, Iaccarino L, Doria A. Genes, epigenetic regulation and environmental factors: which is the most relevant in developing autoimmune diseases? Autoimmun Rev 2012; 11: 604-609.
Dąbrowska-Bouta B, Strużyńska L, Chalimoniuk M, Frontczak-Baniewicz M, Sulkowski G. The influence of glutamatergic receptor antagonists on biochemical and ultrastructural changes in myelin membranes of rats subjected to experimental autoimmune encephalomyelitis. Folia Neuropathol 2015; 53: 317-326.
Garbern JY. Pelizaeus-Merzbacher disease: Genetic and cellular pathogenesis. Cell Mol Life Sci 2007; 64: 50-65.
Ghaiad HR, Nooh MM, El-Sawalhi MM, Shaheen AA. Resveratrol Promotes Remyelination in Cuprizone Model of Multiple Sclerosis: Biochemical and Histological Study. Mol Neurobiol 2017; 54: 3219-3229.
Gold R, Hartung HP, Toyka KV. Animal models for autoimmune demyelinating disorders of the nervous system. Mol Med Today 2000; 6: 88-91.
Golub MS, Germann SL, Lloyd KC. Behavioral characteristics of a nervous system-specific erbB4 knock-out mouse. Behav Brain Res 2004; 153: 159-170.
Greer JM, Lees MB. Myelin proteolipid protein – the first 50 years. Int J Biochem Cell Biol 2002; 34: 211-215.
Janssen A, Fiebiger S, Bros H, Hertwig L, Romero-Suarez S, Hamann I, Chanvillard C, Bellmann-Strobl J, Paul F, Millward JM, Infante-Duarte C. Treatment of Chronic Experimental Autoimmune Encephalomyelitis with Epigallocatechin-3-Gallate and Glatiramer Acetate Alters Expression of Heme-Oxygenase-1. PLoS One 2015; 10: e0130251.
Kang J, Cheng H, Ji J, Incardona J, Rampe D. In vitro electrocardiographic and cardiac ion channel effects of (-)-epigallocatechin-3-gallate, the main catechin of green tea. J Pharmacol Exp Ther 2010; 334: 619-626.
Kasarełło K, Gadamski R, Piotrowski P, Kurzepa K, Kwiatkowska-Patzer B, Lipkowski AW. Effect of oral administration of pig spinal cord hydrolysate on clinical and histopathological symptoms of experimental allergic encephalomyelitis in rats. Folia Neuropathol 2015; 53: 128-138.
Khalatbary AR, Tiraihi T, Boroujeni MB, Ahmadvand H, Tavafi M, Tamjidipoor A. Effects of epigallocatechin gallate on tissue protection and functional recovery after contusive spinal cord injury in rats. Brain Res 2010; 1306: 168-175.
Kipp M, Clarner T, Dang J, Copray S, Beyer C. The cuprizone animal model: new insights into an old story. Acta Neuropathol 2009; 118: 723-736.
Liuzzi GM, Latronico T, Branà MT, Gramegna P, Coniglio MG, Rossano R, Larocca M, Riccio P. Structure-dependent inhibition of gelatinases by dietary antioxidants in rat astrocytes and sera of multiple sclerosis patients. Neurochem Res 2011; 36: 518-527.
Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 2001; 25: 402-408.
Mähler A, Steiniger J, Bock M, Klug L, Parreidt N, Lorenz M, Zimmermann BF, Krannich A, Paul F, Boschmann M. Metabolic response to epigallocatechin-3-gallate in relapsing-remitting multiple sclerosis: a randomized clinical trial. Am J Clin Nutr 2015; 101: 487-495.
Miller A, Korem M, Almog R, Galboiz Y. Vitamin B12, demyelination, remyelination and repair in multiple sclerosis. J Neurol Sci 2005; 233: 93-97.
Münzel EJ, Williams A. Promoting remyelination in multiple sclerosis-recent advances. Drugs 2013; 73: 2017-2029.
Norton WT, Autilio LA. The lipid composition of purified bovine brain myelin. J Neurochem 1966; 13: 213-222.
O’Gorman C, Lucas R, Taylor B. Environmental risk factors for multiple sclerosis: a review with a focus on molecular mechanisms. Int J Mol Sci 2012; 13: 11718-11752.
Pae M, Wu D. Immunomodulating effects of epigallocatechin-3-gallate from green tea: mechanisms and applications. Food Funct 2013; 4: 1287-1303.
Pasquini LA, Calatayud CA, Bertone Uńa AL, Millet V, Pasquini JM, Soto EF. The neurotoxic effect of cuprizone on oligodendrocytes depends on the presence of pro-inflammatory cytokines secreted by microglia. Neurochem Res 2007; 32: 279-292.
Pogačnik L, Pirc K, Palmela I, Skrt M, Kim KS, Brites D, Brito MA, Ulrih NP, Silva RF. Potential for brain accessibility and analysis of stability of selected flavonoids in relation to neuroprotection in vitro. Brain Res 2016; 1651: 17-26.
Renno WM, Al-Maghrebi M, Alshammari A, George P. (-)-Epigal­locatechin-3-gallate (EGCG) attenuates peripheral nerve dege­neration in rat sciatic nerve crush injury. Neurochem Int 2013; 62: 221-231.
Rosetti CM, Maggio B, Oliveira RG. The self organization of lipids and proteins of myelin at the membrane interface. Molecular factors underlying the microheterogeneity of domain segregation. Biochim Biophys Acta 2008; 1778: 1665-1675.
Semnani M, Mashayekhi F, Azarnia M, Salehi Z. Effects of Green Tea Epigallocatechin-3-Gallate (EGCG) On Proteolipid Protein (PLP) and Oligodendrocyte Transcription Factor 1 (Olig1) Expression in the Cerebral Cortex of Cuprizone Induced Multiple Sclerosis Mice; A Western Blot Study. Caspian J Neurol Sci 2016; 2: 1-9.
Sun AY, Wang Q, Simonyi A, Sun GY. Botanical phenolics and brain health. Neuromolecular Med 2008; 10: 259-274.
Sun Q, Zheng Y, Zhang X, Hu X, Wang Y, Zhang S, Zhang D, Nie H. Novel immunoregulatory properties of EGCG on reducing inflammation in EAE. Front Biosci (Landmark Ed) 2013; 18: 332-342.
Sutherland BA, Rahman RM, Appleton I. Mechanisms of action of green tea catechins, with a focus on ischemia-induced neurodegeneration. J Nutr Biochem 2006; 17: 291-306.
Wang J, Cheng J, Li CR, Ye M, Ma Z, Cai F. Modulation of Ca²+ signals by epigallocatechin-3-gallate(EGCG) in cultured rat hippocampal neurons. Int J Mol Sci 2011; 12: 742-754.
Wang J, Ren Z, Xu Y, Xiao S, Meydani SN, Wu D. Epigallocatechin-3-gallate ameliorates experimental autoimmune encephalomyelitis by altering balance among CD4+ T-cell subsets. Am J Pathol 2012; 180: 221-234.
Woodward KJ. The molecular and cellular defects underlying Pelizaeus-Merzbacher disease. Expert Rev Mol Med 2008; 10: e14.
Xin M, Yue T, Ma Z, Wu FF, Gow A, Lu QR. Myelinogenesis and axonal recognition by oligodendrocytes in brain are uncoupled in Olig1-null mice. J Neurosci 2005; 25: 1354-1365.
Yang CS, Maliakal P, Meng X. Inhibition of carcinogenesis by tea. Annu Rev Pharmacol Toxicol 2002; 42: 25-54.
Quick links
© 2018 Termedia Sp. z o.o. All rights reserved.
Developed by Bentus.
PayU - płatności internetowe