eISSN: 1509-572x
ISSN: 1641-4640
Folia Neuropathologica
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2/2022
vol. 60
 
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abstract:
Original paper

Effects of miR-211-3p/RHBDD1 axis on cell proliferation, cell cycle progression, and epithelial-mesenchymal transition in glioma

Lei Chen
1
,
Xiangyi Wang
1
,
Yude Zhu
1
,
Fuquan Liu
1
,
Laixing Liu
1
,
Ning Jiang
1

1.
Department of Neurosurgery, Inner Mongolia Baogang Hospital, Baotou City, Inner Mongolia Autonomous Region, P.R. China
Folia Neuropathol 2022; 60 (2): 195-209
Online publish date: 2022/06/30
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This study was designed to elucidate the relationship of miR-211-3p and rhomboid domain containing 1 (RHBDD1) in glioma. Here, we first observed that miR-211-3p directly targets the 3˘-UTR of RHBDD1 in glioma cells using dual-luciferase reporter assay, RNA immunoprecipitation (RIP) assay, reverse transcription-quantitative polymerase chain reaction (RT-qPCR), and Western blot analysis. Pearson’s correlation analysis showed that miR-211-3p expression was negatively correlated with RHBDD1 expression in glioma tissues. CCK-8 assay, flow cytometry, and transwell assay were applied to assess cell proliferation, cell cycle distribution, migration, and invasion. The results showed that RHBDD1 knockdown inhibited cell proliferation, cell cycle G1/S transition, migration, and invasion in two glioma cell lines (U87 and LN-229). Knockdown of miR-211-3p obtained opposite results. Moreover, overexpression of RHBDD1 counteracted suppressive effects of miR-211-3p on glioma cells. Furthermore, decreased expression of CDK4, cyclin D1, N-cadherin, and vimentin as well as increased E-cadherin expression induced by miR-211-3p was reversed by RHBDD1 overexpression. Our results suggested that targeting miR-211-3p/RHBDD1 axis might be a novel effective therapeutic target for glioma treatment.
keywords:

glioma, miR-211-3p, RHBDD1, proliferation, EMT

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