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ISSN: 1641-4640
Folia Neuropathologica
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4/2015
vol. 53
 
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abstract:
Original paper

Effects of supplementation with branched chain amino acids and ornithine aspartate on plasma ammonia and central fatigue during exercise in healthy men

Tomasz Mikulski, Jan Dabrowski, Wojciech Hilgier, Andrzej Ziemba, Krzysztof Krzeminski

Folia Neuropathol 2015; 53 (4): 377-386
Online publish date: 2015/12/21
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Introduction: Our previous studies showed only slight improvement in central fatigue, measured indirectly by psychomotor performance, after branched chain amino acids (BCAA) supplementation during various efforts in healthy men. It is hypothesised that hyperammonaemia resulting from amino acids metabolism may attenuate their beneficial effect on psychomotor performance; therefore, the L-ornithine L-aspartate (OA) as an ammonia decreasing agent was used. The aim of this study was to investigate the effectiveness of oral BCAA + OA supplementation to reduce plasma ammonia concentration and enhance psychomotor performance during exhaustive exercise in healthy men.

Material and methods: Eleven endurance-trained men (mean age 32.6 ± 1.9 years) performed two sessions (separated by one week) of submaximal cycloergometer exercise for 90 minutes at 60% of maximal oxygen uptake followed by graded exercise until exhaustion with randomised, double-blind supplementation with a total of 16 g BCAA and 12 g OA (BCAA + OA trial) or flavoured water (placebo trial). Before exercise, during both efforts and after 20 minutes of recovery multiple choice reaction time (MCRT), perceived exertion, heart rate and oxygen uptake were measured and venous blood samples were taken for plasma leucine, valine, isoleucine, ornithine, aspartate, free tryptophan (fTRP), ammonia, lactate and glucose determination.

Results: After ingestion, during both efforts and after 20 minutes of recovery the plasma concentrations of all supplemented amino acids were significantly increased, while the fTRP/BCAA ratio decreased in the BCAA + OA trial more than in the placebo trial. At the end of graded exercise plasma fTRP was lower and MCRT shorter in BCAA + OA than in the placebo trial (p < 0.05). At the end of prolonged exercise the plasma ammonia concentration was higher in BCAA + OA than in placebo trial (p < 0.05). Decreases in plasma ammonia during recovery were significantly higher in BCAA + OA than in the placebo trial. Plasma ammonia positively correlated with the total plasma BCAA and MCRT only in the BCAA + OA trial. The fTRP/BCAA ratio positively correlated with MCRT only in the placebo trial.

Conclusions: Supplementation with BCAA and OA is a useful way to improve MCRT during high-intensity exercise and accelerate the elimination of ammonia at the recovery stage after exercise in healthy young men.

keywords:

hyperammonaemia, tryptophan, brain, psychomotor performance, reaction time, effort

references:
Abid S, Jafri W, Mumtaz K, Islam M, Abbas Z, Shah HA, Hamid S. Efficacy of L-ornithine-L-aspartate as an adjuvant therapy in cirrhotic patients with hepatic encephalopathy. J Coll Physicians Surg Pak 2011; 21: 666-671.
Acharya SK, Bhatia V, Sreenivas V, Khanal S, Panda SK. Efficacy of L-ornithine L-aspartate in acute liver failure: a double-blind, randomized, placebo-controlled study. Gastroenterology 2009; 136: 2159-2168.
Albrecht J, Norenberg MD. Glutamine: a Trojan horse in ammonia neurotoxicity. Hepatology 2006; 44: 788-794.
Ament W, Huizenga JR, Kort E, van der Mark TW, Grevink RG, Verkerke GJ. Respiratory ammonia output and blood ammonia concentration during incremental exercise. Int J Sports Med 1999; 20: 71-77.
Areces F, Salinero JJ, Abian-Vicen J, Gonzalez-Millan C, Gallo- Salazar C, Ruiz-Vicente D, Lara B, Del Coso J. A 7-day oral supplementation with branched-chain amino acids was ineffective to prevent muscle damage during a marathon. Amino Acids 2014; 46: 1169-1176.
Bachmann C. Mechanisms of hyperammonemia. Clin Chem Lab Med 2002; 40: 653-662.
Bai M, Yang Z, Qi X, Fan D, Han G. L-ornithine-l-aspartate for hepatic encephalopathy in patients with cirrhosis: a meta-analysis of randomized controlled trials. J Gastroenterol Hepatol 2013; 28: 783-792.
Bai M, He C, Yin Z, Niu J, Wang Z, Qi X, Liu L, Yang Z, Guo W, Tie J, Bai W, Xia J, Cai H, Wang J, Wu K, Fan D, Han G. Randomised clinical trial: L-ornithine-L-aspartate reduces significantly the increase of venous ammonia concentration after TIPSS. Aliment Pharmacol Ther 2014; 40: 63-71.
Banister EW, Cameron BJ. Exercise-induced hyperammonemia: peripheral and central effects. Int J Sports Med 1990; 11: S129-142.
Batra A, Vyas S, Gupta J, Gupta K, Hada R. A comparative study between young and elderly indian males on audio-visual reaction time. Indian Journal of Scientific Research and Technology 2014; 2: 25-29.
Blanco Vela CI, Poo Ramírez JL. Efficacy of oral L-ornithine L-aspartate in cirrhotic patients with hyperammonemic hepatic encephalopathy. Ann Hepatol 2011; 10 Suppl 2: S55-59.
Blomstrand E, Hassmen P, Ekblom B, Newsholme EA. Influence of ingesting a solution of branched-chain amino acids on perceived exertion during exercise. Acta Physiol Scand 1997; 159: 41-49.
Blomstrand E, Perrett D, Parry-Billings M, Newsholme EA. Effect of sustained exercise on plasma amino acid concentrations and on 5-hydroxytryptamine metabolism in six different brain regions of the rat. Acta Physiol Scand 1989; 136: 473-481.
Blomstrand E, Saltin B. BCAA intake affects protein metabolism in muscle after but not during exercise in humans. Am J Physiol Endocrinol Metab 2001; 281: E365-374.
Blomstrand E. A role for branched-chain amino acids in reducing central fatigue. J Nutr 2006; 136: S544-547.
Blomstrand E. Amino acids and central fatigue. Amino Acids 2001; 20: 25-34.
Boado RJ, Li JY, Nagaya M, Zhang C, Pardridge WM. Selective expression of the large neutral amino acid transporter at the blood-brain barrier. Proc Natl Acad Sci USA 1999; 96: 12079-12084.
Borgenvik M, Nordin M, Mikael Mattsson C, Enqvist JK, Blomstrand E, Ekblom B. Alterations in amino acid concentrations in the plasma and muscle in human subjects during 24 h of simulated adventure racing. Eur J Appl Physiol 2012; 112: 3679-3688.
Chaouloff F. Effects of acute physical exercise on central serotonergic systems. Med Sci Sports Exerc 1997; 29: 58-62.
Chmura J, Krysztofiak H, Ziemba AW, Nazar K, Kaciuba-Uściłko H. Psychomotor performance during prolonged exercise above and below the blood lactate threshold. Eur J Appl Physiol Occup Physiol 1998; 77: 77-80.
Chmura J, Nazar K. Kaciuba-Uściłko H. Próg psychomotoryczny zmęczenia. Sport Wyczynowy 2007; 4-6: 508-510.
Chmura J, Nazar K. Parallel changes in the onset of blood lactate accumulation (OBLA) and threshold of psychomotor performance deterioration during incremental exercise after training in athletes. Int J Psychophysiol 2010; 75: 287-290.
Choi S, DiSilvio B, Fernstrom MH, Fernstrom JD. Oral branched-chain amino acid supplements that reduce brain serotonin during exercise in rats also lower brain catecholamines. Amino Acids 2013; 45: 1133-1142.
Curzon G, Friedel J, Knott PJ. The effect of fatty acids on the binding of tryptophan to plasma protein. Nature 1973; 242: 198-200.
Dalsgaard MK, Volianitis S, Yoshiga CC, Dawson EA, Secher NH. Cerebral metabolism during upper and lower body exercise. J Appl Physiol 2004; 97: 1733-1739.
Davis JM, Alderson NL, Welsh RS. Serotonin and central nervous system fatigue: nutritional considerations. Am J Clin Nutr 2000; 72: S573-578.
Du L, Bakish D, Hrdina PD. Tryptophan hydroxylase gene 218A/C polymorphism is associated with somatic anxiety in major depressive disorder. J Affect Disord 2001; 65: 37-44.
Fernstrom JD. Branched-chain amino acids and brain function. J Nutr 2005; 135: S1539-1546.
Greer BK, White JP, Arguello EM, Haymes EM. Branched-chain amino acid supplementation lowers perceived exertion but does not affect performance in untrained males. J Strength Cond Res 2011; 25: 539-544.
Grrishma B, Gaur G S, Velkumary S, Gurunandan U, Dutt A, Dinesh T. Comparison of hand and foot reaction times among females-a methodological study using recognition auditory reaction time. International Journal of Current Research 2013; 5: 4272-4274.
Guezennec CY, Abdelmalki A, Serrurier B, Merino D, Bigard X, Berthelot M, Pierard C, Peres M. Effects of prolonged exercise on brain ammonia and amino acids. Int J Sports Med 1998; 19: 323-327.
Hamon M, Bourgoin S, Artaud F, El Mestikawy S. The respective roles of tryptophan uptake and tryptophan hydroxylase in the regulation of serotonin synthesis in the central nervous system. J Physiol 1981; 77: 269-279.
Haussinger D. Nitrogen metabolism in liver: structural and functional organization and physiological relevance. Biochem J 1990; 267: 281-290.
Hellsten Y, Richter EA, Kiens B, Bangsbo J. AMP deamination and purine exchange in human skeletal muscle during and after intense exercise. J Physiol 1999; 3: 909-920.
Jalan R, Wright G, Davies NA, Hodges SJ. L-Ornithine phenylacetate (OP): a novel treatment for hyperammonemia and hepatic encephalopathy. Med Hypotheses 2007; 69: 1064-1069.
Kilpatrick IC. Rapid, automated HPLC analysis of neuroactive and other amino acids in microdissected brain regions and brain slice superfusates using fluorimetric detection. In: Neuroendocrine research methods. Vol. 2. Greenstein B (ed.). Harwood Academic Publishers, Chur, 1991; pp. 555-578.
MacGillivray L, Reynolds KB, Rosebush PI, Mazurek MF. The comparative effects of environmental enrichment with exercise and serotonin transporter blockade on serotonergic neurons in the dorsal raphe nucleus. Synapse 2012; 66: 465-470.
Meeusen R, Watson P. Amino acids and the brain: do they play a role in “central fatigue”? Int J Sport Nutr Exerc Metab 2007; 17: S37-46.
Mikulski T, Ziemba A, Chmura J, Wiśnik P, Kurek Z, Kaciuba- Uściłko H, Nazar K. The effect of supplementation with branched chain amino acids (BCAA) on psychomotor performance during graded exercise in human subjects. Biol Sport 2002; 19: 295-301.
Nybo L, Dalsgaard MK, Steensberg A, Møller K, Secher NH. Cerebral ammonia uptake and accumulation during prolonged exercise in humans. J Physiol 2005; 563: 285-290.
Parise G, Bosman MJ, Boecker DR, Barry MJ, Tarnopolsky MA. Selective serotonin reuptake inhibitors: Their effect on high-intensity exercise performance. Archives of Physical Medicine and Rehabilitation 2001; 82: 867-871.
Pérez Hernández JL, Higuera de la Tijera F, Serralde-Zúñiga AE, Abdo Francis JM. Critical analysis of studies evaluating the efficacy of infusion of L-ornithine L-aspartate in clinical hepatic encephalopathy in patients with liver failure. Ann Hepatol. 2011; 10 Suppl 2: S66-69.
Pitkanen H, Nykanen T, Knuutinen J. Free amino acid pool and muscle protein balance after resistance exercise. Med Sci Sports Exerc 2003; 35: 784-792.
Rees CJ, Oppong K, Al Mardini H, Hudson M, Record CO. Effect of L-ornithine-L-aspartate on patients with and without TIPS undergoing glutamine challenge: a double blind, placebo controlled trial. Gut 2000; 47: 571-574.
Schmid M, Peck-Radosavljevic M, König F, Mittermaier C, Gangl A, Ferenci P. A double-blind, randomized, placebo-controlled trial of intravenous L-ornithine-L-aspartate on postural control in patients with cirrhosis. Liver Int 2010; 30: 574-582.
Shephard RJ, Johnson N. Effects of physical activity upon the liver. Eur J Appl Physiol 2015; 115: 1-46.
Sikorska H, Cianciara J, Wiercińska-Drapało A. Physiological functions of L-ornithine and L-aspartate in the body and the efficacy of administration of L-ornithine-L-aspartate in conditions of relative deficiency. Pol Merkur Lekarski 2010; 28: 490-495.
Staedt U, Leweling H, Gladisch R, Kortsik C, Hagmüller E, Holm E. Effects of ornithine aspartate on plasma ammonia and plasma amino acids in patients with cirrhosis. A double-blind, randomized study using a four-fold crossover design. J Hepatol 1993; 19: 424-430.
Strüder HK, Hollmann W, Platen P, Donike M, Gotzmann A, Weber K. Influence of paroxetine, branched-chain amino acids and tyrosine on neuroendocrine system responses and fatigue in humans. Horm Metab Res 1998; 30: 188-194.
Strüder HK, Weicker H. Physiology and pathophysiology of the serotonergic system and its implications on mental and physical performance. Int J Sports Med 2001; 22: 482-497.
Wagenmakers AJ, Coakley JH, Edwards RH. Metabolism of branched-chain amino acids and ammonia during exercise: clues from McArdle’s disease. Int J Sports Med 1990; 11 Suppl 2: S101-113.
Wagenmakers AJ, Beckers EJ, Brouns F, Kuipers H, Soeters PB, van der Vusse GJ, Saris WH. Carbohydrate supplementation, glycogen depletion, and amino acid metabolism during exercise. Am J Physiol 1991; 260 (6 Pt 1): E883-890.
van Wijck K, Lenaerts K, van Loon LJ, Peters WH, Buurman WA, Dejong CH. Exercise-induced splanchnic hypoperfusion results in gut dysfunction in healthy men. PLoS One 2011; 6: e22366.
Wiśnik P, Chmura J, Ziemba AW, Mikulski T, Nazar K. The effect of branched chain amino acids on psychomotor performance during treadmill exercise of changing intensity simulating a soccer game. Appl Physiol Nutr Metab 2011; 36: 856-862.
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