eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
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1/2015
vol. 40
 
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abstract:

Experimental immunology
Proteomic and immunological identification of two new allergens from silkworm (Bombyx mori L.) pupae

Xiangjie Zhao
,
Lin Li
,
Zheshi Kuang
,
Guoqing Luo
,
Bing Li

(Centr Eur J Immunol 2015; 40 (1): 30-34)
Online publish date: 2015/04/22
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This study explored food allergy caused by eating silkworm (Bombyx mori L.) pupae, a traditionally accepted food and animal feed in East and Southeast Asia, and identified two new allergens by proteomic and immunological methods. Proteins isolated from silkworm pupae were separated by two-dimensional gel electrophoresis (2-DE); pooled sera from patients allergic to silkworm pupa proteins were used to detect immunoglobulin E (IgE)-binding proteins by western blotting, and allergens specific for silkworm pupa consumption-caused allergy were visualised with the ECL reagents. The selected allergen proteins were further identified by matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF-MS) analysis. Finally, chitinase and paramyosin were identified as silkworm pupa proteins showing strong immunoglobulin (IgE)-binding reaction. Analysis of the sequence homology of the two proteins using the AllergenOnline database indicated that chitinase and paramyosin shared 24.8% and 62.8% sequence homology with known allergens Der f 18 (Dermatophagoides farinae) and Der p 11 (Dermatophagoides pteronyssinus), respectively. Our results shed light on the understanding and treatment of silkworm pupa allergy.
keywords:

silkworm (Bombyx mori L) pupa, allergen, chitinase, paramyosin, western blot, MALDI-TOF-MS

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