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Folia Neuropathologica
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vol. 61
Original paper

Knockdown of lncRNA BDNF-AS alleviates isoflurane-induced neuro-inflammation and cognitive dysfunction through modulating miR-214-3p

Lin Wang
Yajun Mao
2, 3
Yugang Lu
Yawei Yuan
Yanwu Jin

Department of Anesthesiology, Guangzhou Red Cross Hospital (Guangzhou Red Cross Hospital of Jinan University), Guangzhou, China
Department of Operating Room, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China
Department of Nursing, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China
Department of Anesthesiology, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China
Department of Anesthesiology, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai, China
Department of Anesthesiology, The Second Hospital of Shandong University, Shandong University, Jinan, China
Folia Neuropathol 2023; 61 (1): 68-76
Online publish date: 2023/02/17
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As one of the most commonly used anesthetics, isoflurane has been demonstrated to possess a variety of protective effects. However, its’ neurological impaired effect should be considered during clinical application. Roles of lncRNA BDNF-AS (BDNF-AS) and miR-214-3p in isoflurane-injured microglia and rats were investigated in this study, aiming to disclose the mechanism of isoflurane damage and to provide candidate therapeutic targets.

Material and methods:
Isoflurane-induced microglia cells and rat models were established with 1.5% isoflurane. Inflammation and oxidative stress of microglia cells were evaluated with a level of pro-inflammation cytokines, malondialdehyde (MDA), superoxide dismutase (SOD), and nitrite. Cognitive and learning function of rats were assessed with Morris water maze task. Expressions of BDNF-AS and miR-214-3p and their function in the isoflurane-induced microglia cells and rats were estimated with PCR and corresponding transfection.

Isoflurane induced significant neuro-inflammation and oxidative stress in the microglia cells. The increased BDNF-AS and the decreased miR-214-3p were noted, and BDNF-AS was found to negatively regulate miR-214-3p in the isoflurane-induced microglia cells. Isoflurane caused cognitive dysfunction in rats, and resulted in a significant inflammatory response. The knockdown of BDNF-AS significantly alleviated the neurological impairment induced by isoflurane, which was reversed by silencing miR-214-3p.

In isoflurane-induced neuro-inflammation and cognitive dysfunction, BDNF-AS showed a significant protective effect on the neurological impairment induced by isoflurane through modulating miR-214-3p.


isoflurane, lncRNA BDNF-AS, miR-214-3p, neuro-inflammation, cognitive and learning dysfunction

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