eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
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SCImago Journal & Country Rank
2/2019
vol. 44
 
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abstract:
Clinical immunology

MiR-214 regulates CD3ζ expression in T cells

Yankai Xiao
1, 2
,
Lixing Guo
2
,
Suwen Zhao
1, 2
,
Guixuan Huang
1, 2
,
Shaohua Chen
2
,
Lijian Yang
2
,
Yangqiu Li
1, 2, 3
,
Bo Li
1, 2

1.
Key Laboratory for Regenerative Medicine of Ministry of Education, Jinan University, Guangzhou, China
2.
Institute of Haematology, School of Medicine, Jinan University, Guangzhou, China
3.
Department of Haematology, First Hospital Affiliated, Jinan University, Guangzhou, China
(Centr Eur J Immunol 2019; 44 (2): 127-131)
Online publish date: 2019/07/30
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Introduction
T-cell activation requires the T-cell receptor (TCR)-CD3 complex, which integrates and transduces signals. CD3 plays a vital role in TCR signalling by mediating T-cell activation. Abnormal CD3 expression is a common characteristic of haematological malignancies with T-cell immune dysfunction or autoimmune diseases. Targeted regulation of CD3 expression by either direct or indirect approaches is important for regulating T-cell activation.

Aim of the study
In this study, we focused on identifying miRNAs that may regulate CD3 expression.

Material and methods
Three microRNA target search algorithms (TargetScan, PicTar, and microrna.org) were used to identify hypothetical miRNAs that target CD3 in T cells. Of the predicted miRNAs, miR-214 was chosen and validated to determine whether miR-214 directly binds to the CD3 3’-UTR and regulates CD3 expression by luciferase reporter assays, real-time PCR, and Western blotting.

Results
The results indicate that miR-214 specifically binds the CD3 3’-UTR, and miR-214 mimics remarkably reduce the expression of CD3 in MOLT-4 cells.

Conclusions
We identify for the first time that miR-214 targets expression in MOLT-4 cells, suggesting that miR-214 might negatively regulate T-cell activation by targeting CD3.

keywords:

T cell, CD3, miR-214

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