Abstract
1/2006
vol. 44
ORIGINAL ARTICLEThe presence of endotoxin in synovial fluids from connective tissue diseases (Ctd-s) patients with as a suspected disease aethiological marker
RU 2006; 44, 1: 26-33
Online publish date: 2006/02/15
The aim of the study was to show the presence of endotoxin in the synovial fluids from the patients suffering of Ctd-s (in prevalence RA and other undifferentiated joint arthritis).
The usefulness of the applied LAL-test (Limulus Amebocyte Lysate) was evaluated (just in endotoxin /LPS/ assessment), as a main and functionally most important of the Gram-negative bacterial cell wall component.
It was proved, that in the group of 50 synovial fluids from Ctd-s patients 42% was LAL-positive (with presence of endotoxin) and in 60% of RA patients LAL-test was also positive.
Simultaneously, in the total amount of LAL-negative synovial fluids we found, that in 66.6% the inhibiting LAL-test substances (including antibody) have been found. It suggest, that in tested material we have obtained considerable amount of the fals-negative tests (results), because that a retrospective analysis showed in tested synovial fluids the presence of anti-LPS or antibodies to selected LPS subcomponents up to 80%.
In discussion a possible influence of endotoxin, especially bacilli from genus Salmonella enteritidis (or S. typhimurium) and their toxic component (lipid A) on the induction acute phase Ctd-s (especially in RA and undifferentiated inflammation of joints) – basing on our own experiments and literature, was touched.
The usefulness of the applied LAL-test (Limulus Amebocyte Lysate) was evaluated (just in endotoxin /LPS/ assessment), as a main and functionally most important of the Gram-negative bacterial cell wall component.
It was proved, that in the group of 50 synovial fluids from Ctd-s patients 42% was LAL-positive (with presence of endotoxin) and in 60% of RA patients LAL-test was also positive.
Simultaneously, in the total amount of LAL-negative synovial fluids we found, that in 66.6% the inhibiting LAL-test substances (including antibody) have been found. It suggest, that in tested material we have obtained considerable amount of the fals-negative tests (results), because that a retrospective analysis showed in tested synovial fluids the presence of anti-LPS or antibodies to selected LPS subcomponents up to 80%.
In discussion a possible influence of endotoxin, especially bacilli from genus Salmonella enteritidis (or S. typhimurium) and their toxic component (lipid A) on the induction acute phase Ctd-s (especially in RA and undifferentiated inflammation of joints) – basing on our own experiments and literature, was touched.
Keywords
endotoxin, Limulus Amebocyte Lysate (LAL) test, lipopolysaccharide, synovial fluid
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