eISSN: 1509-572x
ISSN: 1641-4640
Folia Neuropathologica
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SCImago Journal & Country Rank
2/2018
vol. 56
 
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abstract:
Original paper

Pseudopterosin A ameliorates ischaemia-induced brain injury by acting on Akt signalling pathway

Xinkai Niu, Chaochun Yu, Guanfu Jiang, Jun Wu, Xiaohui Tan, Weijia Zhang, Lijun Hou

Folia Neuropathol 2018; 56 (2): 104-111
Online publish date: 2018/06/28
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Introduction
Brain injury caused by ischaemic stroke is a major cause of disability and death throughout the world. The present study evaluates the neuroprotective effect of pseudopterosin A (PtA) against ischaemia-induced brain injury.

Material and methods
Ischaemia was induced by pMCAO model, and rats were separated in to three groups. A control group; an ischemic group receiving saline solution; and a PtA group receiving PtA (5 mg/kg, i.p.) for the period of three days, i.e. two days before surgery and after the surgery. Effect of PtA was assessed by estimating the infract volume and neurological deficits. Level of oxidative stress, inflammatory mediators, and markers of apoptosis were estimated in the brain tissues. Western blot assay and immunohistochemistry was done for the assessment of expressions of protein.

Result
Data from the study suggest that treatment with PtA significantly decreases the infracted volume and neurological deficit score compared to the ischaemic group. Treatments with PtA attenuate the activity of antioxidant enzyme, and the level of inflammatory mediators and markers of apoptosis in the brain tissues of ischaemia-induced brain injury. Phosphorylation of FKHR, Bad, and Akt were significantly reduced in the PtA-treated group compared to the ischaemic group.

Conclusions
The present study shows that pseudopterosin A attenuates neuronal injury in the pMCAO model by acting on the Akt signalling pathway.

keywords:

pseudopterosin A, brain injury, Akt signalling, apoptosis

references:
Alberts B, Johnson A, Lewis J, Raff M, Roberts K, Walter P. Molecular Biology of the Cell. 4th edition. Programmed Cell Death (Apoptosis). Garland Science, New York 2002.
Bhattacharyya A, Chattopadhyay R, Mitra S, Crowe SE. Oxidative stress: an essential factor in the pathogenesis of gastrointestinal mucosal diseases. Physiol Rev 2014; 94: 329-354.
Caplan SL, Zheng B, Scully KD, White CA, West LM. Pseudo­pterosin A: protection of synaptic function and potential as a neuromodulatory agent. Mar Drugs 2016; 14: E55.
Chin JH, Vora N. The global burden of neurologic diseases. Neurology 2014; 83: 349-351.
González Y, Mendoza DT, Jones GE, Fernandez PL, Marine diterpenoids as potential anti-inflammatory agents. Mediators Inflamm 2015; 2015: 263543.
Hoffer BJ, Pick CG, Hoffer ME, Becker RE, Chiang YH, Greig NH. Repositioning drugs for traumatic brain injury – N-acetyl cysteine and Phenserine. J Biomed Sci 2017; 24: 71.
Jin R, Yang G, Li G. Inflammatory mechanisms in ischemic stroke: role of inflammatory cells. J Leukoc Biol 2010; 87: 779-789.
Kohl AC, Kerr RG. Pseudopterosin biosynthesis: aromatization of the diterpene cyclase product, Elisabethatriene. Mar Drugs 2003; 1: 54-65.
Kohl AC, Kerr RG. Identification and characterization of the pseudopterosin diterpene cyclase, elisabethatriene synthase, from the marine gorgonian, Pseudopterogorgia elisabethae. Arch Biochem Biophys 2004; 424: 97-104.
Kong X, Gong S, Su L, Li C, Kong Y. Neuroprotective effects of allicin on ischemia-reperfusion brain injury. Oncotarget 2017; 8: 104492-104507.
Li X, Zhang J, Zhu X, Wang P, Wang X, Li D. Progesterone reduces inflammation and apoptosis in neonatal rats with hypoxic ischemic brain damage through the PI3K/Akt pathway. Int J Clin Exp Med 2015; 8: 8197-8203.
Liu S, Sun Z, Chu P, Li H, Ahsan A, Zhou Z, Zhang Z, Sun B, Wu J, Xi Y, Han G, Lin Y, Peng J, Tang Z. EGCG protects against homocysteine-induced human umbilical vein endothelial cells apoptosis by modulating mitochondrial-dependent apoptotic signaling and PI3K/Akt/eNOS signaling pathways. Apoptosis 2017; 22: 672-680.
Malve H. Exploring the ocean for new drug developments: Marine pharmacology J Pharm Bioallied Sci 2016; 8: 83-91.
McCulloch MWB, Haltli B, Marchbank DH, Kerr RG. Evaluation of pseudopteroxazole and pseudopterosin derivatives against Mycobacterium tuberculosis and other pathogens. Mar Drugs 2012; 10: 1711-1728.
Moya CE, Jacobs RS. Pseudopterosins appear to enhance the wound healing process through an adenosine receptor mediated mechanism. FASEB J 2008; 22 Suppl 1: 729.7-729.7.
Nour M, Scalzo F, Liebeskind DS. Ischemia-reperfusion injury in stroke. Interv Neurol 2013; 1: 185-199.
Pap M, Cooper GM. Role of translation initiation factor 2B in control of cell survival by the phosphatidylinositol 3-kinase/Akt/glycogen synthase kinase 3 signaling pathway. Mol Cell Biol 2002; 22: 578-586.
Quillinan N, Herson PS, Traystman RJ. Neuropathophysiology of brain injury. Anesthesiol Clin 2016; 34: 453-464.
Sabirzhanov B, Stoica BA, Zhao Z, Loane DJ, Wu J, Dorsey SG, Faden AI. miR-711 upregulation induces neuronal cell death after traumatic brain injury. Cell Death Differ 2016; 23: 654-668.
Sperlich J, Kerr R, Teusch N, The marine natural product pseudopterosin blocks cytokine release of triple-negative breast cancer and monocytic leukemia cells by inhibiting NF-B signalling. Mar Drugs 2017; 15: 262.
Wang Z, Zhou L, Zheng X, Chen G, Pan R, Li J, Liu W. Autophagy protects against PI3K/Akt/mTOR-mediated apoptosis of spinal cord neurons after mechanical injury. Neurosci Lett 2017; 656: 158-164.
Xu M, Zhang H. Death and survival of neuronal and astrocytic cells in ischemic brain injury: a role of autophagy. Acta Pharmacol Sin 2011; 32: 1089-1099.
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