eISSN: 1509-572x
ISSN: 1641-4640
Folia Neuropathologica
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SCImago Journal & Country Rank
2/2012
vol. 50
 
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abstract:

Original article
Differential expression of MMP-9 and AQP4 in human glioma samples

Wei-Jiang Zhao
,
Wei Zhang
,
Gui-Lin Li
,
Yun Cui
,
Zhong-Fang Shi
,
Fang Yuan

Folia Neuropathol 2012; 50 (2): 176-186
Online publish date: 2012/06/27
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Background: Metalloproteinase-9 (MMP-9) and aquaporin-4 (AQP4) have been individually reported in glioma development. Here, we co-analyzed their expression in multiple forms of human glioma tissues graded from II to IV.

Material and methods: Levels of MMP-9 and AQP4 were evaluated on 50 resected human glioma tissues using immunohistochemistry. Protein levels of both molecules were evaluated by a staining score system based on the percentage of positive cells/staining degree in each dot section. The transwell method was also used to discriminate fast migrating cells and slow migrating cells, in which expression of both MMP-9 and AQP4 was investigated by using immunofluorescence.

Results: The staining score of MMP-9 displayed a positively tumor grade dependent manner, whereas AQP4 expression showed a negatively tumor grade dependent manner. The nuclear translocation of both molecules was observed in astrocytomas with glioblastoma transition, or glioblastoma tissues. Fast migrating cells contain more AQP4, whereas more MMP-9 was localized in slow migrating cells.

Conclusions: Our findings suggest differential expression patterns of MMP-9 and AQP4 in different grades of gliomas. Nuclear translocation of MMP-9 and AQP4 may exert more functions in glioblastoma transition or deterioration.
keywords:

metalloproteinase-9 (MMP-9), aquaporin-4 (AQP4), human glioma

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