eISSN: 1897-4317
ISSN: 1895-5770
Gastroenterology Review/Przegląd Gastroenterologiczny
Current issue Archive Manuscripts accepted About the journal Editorial board Abstracting and indexing Subscription Contact Instructions for authors Ethical standards and procedures
SCImago Journal & Country Rank
vol. 6
Original paper

Different effectiveness of Helicobacter pylori lipopolysaccharides with or without LewisXY determinants in stimulating the secretion of proinflammatory cytokines IL-8 and TNF-α by peripheral blood mononuclear leukocytes

Karolina Rudnicka
Aneta Grębowska
Anthony P. Moran
Agnieszka Matusiak
Maria Walencka
Eliza Miszczyk
Leokadia Bąk-Romaniszyn
Elżbieta Czkwianianc
Izabela Płaneta-Małecka
Wiesława Rudnicka
Magdalena Chmiela

Przegląd Gastroenterologiczny 2011; 6 (6): 401–408
Online publish date: 2011/12/03
View full text
Get citation
JabRef, Mendeley
Papers, Reference Manager, RefWorks, Zotero
Introduction: Helicobacter pylori is an aetiological agent of chronic gastritis, gastric and duodenal ulcers, and gastric cancers. It is suggested that H. pylori must have undergone evolutionary changes that enable the bacteria to overcome the host immune response. The molecular mimicry between Lewis (Le) determinants present in H. pylori lipopolysaccharide (LPS) and on the host cells may play a role in the outcome of H. pylori infections.

Aim: In this study we investigated the production of inflammatory cytokines tumour necrosis factor α (TNF-α) and interleukin 8 (IL-8), by human peripheral blood mononuclear leukocytes (PBML) from H. pylori infected (11) and uninfected (10) subjects (21 women, aged 25-50 years), in response to H. pylori-LPS with LeXY(+) or without LeXY(–) determinants.

Material and methods: Peripheral blood was collected using the Vacutainer heparin system and constituted a source of total or monocyte and lymphocyte enriched PBML fractions. Tumour necrosis factor α and IL-8 were assessed by immunosorbent assay (ELISA) in the supernatants from leukocyte cultures stimulated or not with H. pylori LPS or standard Escherichia coli LPS.

Results: The results showed that adherent but not non-adherent PBML responded to H. pylori -LPS by TNF- α and IL-8 production. The H. pylori -LPS LeXY(+) was a stronger stimulus for macrophage-derived cytokines, as compared with H. pylori -LPS LeXY(–). Helicobacter pylori-LPS LeXY(+) driven production of TNF- α and IL-8 was significantly inhibited with antibodies to LPS-binding protein (LBP) and to CD14 proteins.

Conclusions: It is possible that LeXY determinants of H. pylori -LPS could be involved in LPS signalling for inflammatory cytokines and thus regulate the outcome of H. pylori infections.

Helicobacter pylori, lipopolysaccharides, interleukin 8, tumour necrosis factor α

Quick links
© 2019 Termedia Sp. z o.o. All rights reserved.
Developed by Bentus.
PayU - płatności internetowe